Part:BBa_K1642010:Experience

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Applications of BBa_K1642010

Based on PcpcG2 and halorhodopsin, we established a biodesalination system which relies on red and green light. The light colors in the three stages are red light, green light and white light respectively.

In the growth stage, red light is enough for photosynthesis of cyanobacteria, which has been proved to be feasible by a recent report(Abe, K. et al. 2014). To further confirm it, we determined the growth curve under red light. This growth rate is no slower than that of the stain under natural light as long as we adjust the light intensity.

In the induction stage, the green light induces the expression of HR. Additionally, green light can’t provide energy source for chlorophyll, thus creating a starvation condition. And the starvation can inhibit active export of sodium, which is essential for biodesalination in the working stage.

In the working stage, halorhodopsin absorbs light to drive chloride import. Addtionally, cyanobacteria will regain energy under natural light. The biodesalination process controlled by PcpcG2 is shown in Figure 1.

The effectiveness of this biodesalinaion process is proved by determination of the concentrations of extracelluar sodium and chloride or desalination assay, which are shown in Figure 2. During the early time of working stage, there is an obvious decrease of concentration compared to that of Wild-type, which indicates that our biobrick (PcpcG2-HR, BBa_K1642010) really works in cyanobacteria under this biodesalination process! The following rise of concentration can be explained by the regain of energy under natural light in the working stage. Considering that we focus on the biodesalination process controlled by Pdark, we didn’t optimize this process.

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