Generator

Part:BBa_K1713004

Designed by: Xudong Luo   Group: iGEM15_HZAU-China   (2015-09-14)
Revision as of 01:14, 19 September 2015 by Luoxudong (Talk | contribs)

Plac/ara-1+strong RBS+mRFP+double terminator

The hybrid promoter ( Plac/ara-1 ) can be activated by the AraC protein in the presence of arabinose and so that mRFP produced. And then the LacI protein would repress this hybrid promoter ( Plac/ara-1 ) in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 683
    Illegal AgeI site found at 795
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This device is composed with the hybrid promoter(Plac/ara-1) and a mRFP linked behind it. Some related tests are specifically run under two different chemical inducers(Arabinose/IPTG) respectively. A series of different inducer's concentrations are set to test amples, and the expressions level of mRFP is linked with the influence that changed concentration of inducer had on the promoter. Related data after analysis show as the following charts.

Team_HZAU-China_Fig_W13.png Fig 7:The effect on the promoter of Arabinose. Team_HZAU-China_Fig_W14.png Fig 8:The effect on the promoter of IPTG.

Based on results above, this device reveals to be activated by the AraC protein in the presence of arabinose and repressed in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG).

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Categories
Parameters
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