Part:BBa_K1649003

YF1 blue light sensor with GFP reporter

GFP is used to report the function of blue light sensor. The LOV photo-switch YF1 was created by fusing the light perception domain of YtvA to the FixL kinase domain. YtvA acts as a blue-light photoreceptor and carries a blue-light-sensitive flavin-binding LOV domain. The response regulator FixJ forms a two-component system together with FixL.The sensor is inactive under blue light and active in dark. [[File:===Measurement===

In order to test whether this part works,we cloned this part into pET28a using the NotI and XbaI sites. This plasmid contains a Kanamycin resistance gene and a pBR322 origin. BL21 strains are used to harbor blue light sensor plasmid.We measured OD600 and fluorescence of the strains at intervals.

[[file:‪BBa_K1649003.png] ]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 605
  Illegal NgoMIV site found at 677
  Illegal NgoMIV site found at 767
  Illegal NgoMIV site found at 785
  Illegal NgoMIV site found at 1297
  Illegal NgoMIV site found at 1590
  Illegal NgoMIV site found at 1684
  Illegal AgeI site found at 319
  Illegal AgeI site found at 1465
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1354
  Illegal BsaI.rc site found at 218
  Illegal BsaI.rc site found at 2904
  

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