Generator

Part:BBa_K1807004

Designed by: Ivan Gyulev   Group: iGEM15_York   (2015-09-17)
Revision as of 01:03, 19 September 2015 by Isgyulev (Talk | contribs) (→‎Usage and Biology)

Polyphosphate kinase gene from Accumulibacter phosphatis SK-12

This part is a device producing polyphosphate kinase enzyme from Candidatus Accumulibacter phosphatis SK-12.

Usage and Biology


The known Candidatus Accumulibacter phosphatis strains are considered Polyphosphate-Accumulating Organisms (PAOs). They were first discovered in Activated Sludge performing Enhanced Biological Phosphorus Removal in wastewater treatment facilities. Those organisms are capable of accumulating more than two times higher percentage of their dry weight in phosphorus than their non-PAO counterparts. Unfortunately, Accumulibacter has so far proven unculturable which makes it difficult to examine its genetics and biochemistry. However, metagenomic sequences from enriched cultures have been obtained in recent years and this has enabled the production of phylogenetic analysis and mostly-complete genomic sequence assemblies. iGEM York 2015 decided to tap into the PAOs potential and systematically test Accumulibacter phosphate-uptake and storage related enzymes. Rather than attempt to clone the genes from Activated sludge, iGEM York 2015 designed chemically synthesized DNA based on the metagenomic sequence samples available.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1063
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 128
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 554
    Illegal SapI site found at 714


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Categories
Parameters
None