Generator

Part:BBa_K1631012

Designed by: Yuto Yamanaka   Group: iGEM15_UT-Tokyo   (2015-09-14)
Revision as of 00:34, 19 September 2015 by Yamanaka (Talk | contribs)

Plac -rbs- Barnase -rbs- GFP -d.term

Barnase and GFP is co-expressed by IPTG induction.

If you use this part, you can estimated the expression level of barnase and evaluate its toxic activity correspond to its expression level.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1081


[edit]
Categories
//function/celldeath
Parameters
None