Composite

Part:BBa_K1642009:Design

Designed by: Can Yin   Group: iGEM15_SJTU-BioX-Shanghai   (2015-09-05)
Revision as of 00:33, 19 September 2015 by EvaJiang (Talk | contribs) (→‎Design Notes)

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Expression of GFP under the control of Pdark


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 611
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2995
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1294


Design Notes

Pdark(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Promoters)

Pdark-GFP

Source

ClonExpress technique of Vazyme Biotech Co.,Ltd from composite Pdark-HR(BBa_K1642011) and GFP gene sequence.