Composite

Part:BBa_K1675004

Designed by: JIAQI XU   Group: iGEM15_BIT-China   (2015-09-10)
Revision as of 00:30, 19 September 2015 by JIAQIXU (Talk | contribs)

J23119+B0034+NhaA(BBa_K1675000)

The strong constitutive promoter J23119, the ribosome binding site B0034 and the functional gene NhaA([BBa_K1675000]) were used in this new standard part. This standard part is available for the bacteria to survive in alkaline environment.

We designed suitable primers and linked JBA (J23119+B0034+NhaA) with pSB1C3. The agarose gel electrophoresis analysis below (Fig.1) shows the positive clones of JBA.

Fig.5-a.png

Fig.1 The positive clones of JBA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 324
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 521
    Illegal BamHI site found at 1058
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 384
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
biologyEscherichia coli str. K-12 substr.