Generator

Part:BBa_K1807005:Design

Designed by: Ivan Gyulev   Group: iGEM15_York   (2015-09-17)
Revision as of 00:30, 19 September 2015 by Isgyulev (Talk | contribs) (→‎Design Notes)

Polyphosphate Kinase from Accumulibacter phosphatis UW-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1867
    Illegal BamHI site found at 559
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1775


Design Notes


iGEM York 2015 generated this part by designing two overlapping G Block fragments (999bp and 1245bp) that were used in a Gibson Assembly Reaction with BBa_K1807000.


Source

Candidatus Accumulibacter phosphatis UW-1

References