Composite

Part:BBa_K1813013

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-07-27)
Revision as of 00:30, 19 September 2015 by Ishd (Talk | contribs)

CYP2D6 Expression Cassette

A construct used to achieve the overexpression of HUMCYPDB1/CYP2D6 (BBa_K11813005) in a bacterial host. Structure: ptac:: HUMCYPDB1:: term.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 410
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1000
  • 1000
    COMPATIBLE WITH RFC[1000]



Background

Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of HUMCYPDB1 by a bacterial organism harboring this construct. This construct was assembled by Michael VanInsberghe, and is codon optimized for E. coli .
It is used in BBa_K1813024 and BBa_K1813032.

[edit]
Categories
Parameters
n/aptac CYP2D6 term