Generator

Part:BBa_K1807004:Design

Designed by: Ivan Gyulev   Group: iGEM15_York   (2015-09-17)
Revision as of 00:28, 19 September 2015 by Isgyulev (Talk | contribs) (→‎Design Notes)

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Polyphosphate kinase gene from Accumulibacter phosphatis SK-12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1063
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 128
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 554
    Illegal SapI site found at 714


Design Notes

iGEM York 2015 generated this part by designing two overlapping G Block fragments (999bp and 1245bp) that were used in a Gibson Assembly Reaction with BBa_K1807000.

Source

Candidatus Accumulibacter phosphatis SK-12

References