DNA

Part:BBa_K1614025

Designed by: Daniel Heid   Group: iGEM15_Heidelberg   (2015-09-18)
Revision as of 00:19, 19 September 2015 by Registry (Talk | contribs)

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Ketamin-dependent self cleaving F8 - readout DNAzyme

Construct consisting of a ketamin-switched F8 DNAzyme linked to a stemmed HRP mimicking DNAzyme.

If there no ketamine binds to aptamer the F8 is inactive and HRP mimicking DNAzyme is stemmed, so that no hemin could bind and there is no peroxidase activity.

By binding of ketamin to the aptamer, F8 is activated and cleaves the stemming part, so the HRP mimicking DNAzyme is activated, peroxidase activity could be used for different readout techniques.

Stems and Aptamer generated by MAWS and JAWS software.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None