Generator

Part:BBa_K1807001:Design

Designed by: Ivan Gyulev   Group: iGEM15_York   (2015-09-17)
Revision as of 00:06, 19 September 2015 by Isgyulev (Talk | contribs) (→‎Design Notes)

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Escherichia coli Exopolyphosphatase (PPX)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 872


Design Notes

This part was sub-cloned from the pBC9 plasmid, first described by Akiyama et al., 1993. iGEM York 2015 obtained pBC9 with Dr Jay Keasling's kind assistance.

The primer pair used to PCR clone the part:

Forward: AGCACATACGAGAAAGAGGAGAAATACcccATGCCAATACACGATAAATCCCCT

Reverse: GAGCCTTTCGTTTTATTTGATGCCTGGcccTTAAGCGGCGATTTCTGGTGTACT

Illegal restriction enzyme site removal was unnecessary.

Source

Escherichia coli W3110

References