Part:BBa_K1642004

SD sequence of cpcB

The SD-like sequence derived from the cpcB gene of Synechococcus sp. PCC7002, 5′-UAUAAGUAGGAGAUAAAAAC-3′(Abe, K. et al. 2014), is to be introduced at upstream of the start codon of the genes under the PcpcG2.

Ribosome binding sites(RBS) control protein expression level to a great extent. The PcpcG2 promoter does not have a typical Shine-Dalgarno (SD) sequence upstream of the start codon. Reported weak expression may be due to the lack of a SD-like sequence, so we add an SD sequence on the gene under PcpcG2, hoping that the gene will express on a relatively high level.

Highly expressed photosynthesis-related proteins in cyanobacteria, the cpcB encoded c-phycocyanin, has in their gene’s 5′-UTR the SD-like sequences AGGAG respectively. The SD-like sequence from cpcB is completely complementary to the 3′ region of 16S rRNA of Synechocystis sp. PCC6803 and is highly conserved among various cyanobacteria.

Reported about 15-fold higher GFPuv-derived fluorescence intensity resulted from the addition of the SD-like sequence to PcpcG2 proved that the SD-like sequence function strongly in Synechocystis sp. PCC6803.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]