Generator

Part:BBa_K1807006:Design

Designed by: Ivan Gyulev   Group: iGEM15_York   (2015-09-17)
Revision as of 23:49, 18 September 2015 by Isgyulev (Talk | contribs) (→‎Design Notes)

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Polyphosphate kinase gene from Kingella oralis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 350


Design Notes


The Kingella oralis PPK coding sequence was sub-cloned from the translational unit BBa_K1217003 part.

The method used was PCR amplification with overhang-containing primers, followed by Gibson Assembly with SmaI-cut BBa_K1807000.

The primer pair used to generate the PCR product:

Forward: 5' AGCACATACGAGAAAGAGGAGAAATACCCCATGCCGCAATCTGCTCACATTCTTTGCCGC 3'

Reverse: 5' GAGCCTTTCGTTTTATTTGATGCCTGGCCCTCATTCCGTATATCGCGCCAACAGCGTTTC 3'

Source

Kingella oralis ATCC 51147

References