Coding

Part:BBa_K1585203

Designed by: Carolin Grtering   Group: iGEM15_Aachen   (2015-08-25)
Revision as of 23:45, 18 September 2015 by Pdemling (Talk | contribs) (→‎Usage and Biology)

XPK

D-Xylulose-5-phosphate-phosphoketolase (Xpk) from Bifidobacterium adolescentis, codon optimized for E. coli, converts Xu5p and Pi to actp and G3P


Catalysed reaction by Xpk
D-Xylulose-5-phosphate + phosphate → Acetyl-phosphate + D-Glyceraldehyde-3-phosphate.



Usage and Biology

Team:Aachen/Aachen_15-08-10_Mdh,_Xpk_f._Wiki_2.png

Mdh and Xpk in a pSB1A30 backbone on SDS page after IPTG induction with a RFP expression strain as negative control|subtitle1=all genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction|subtitle2=all genes expressed in BL21 Gold (DE3), 2 ml samples taken from 50 ml main culture in shake flasks after 6h and 21 h after IPTG induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1408
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None