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Part:BBa_K1384000:Experience

Designed by: Anuved Verma   Group: iGEM14_UCLA   (2014-10-08)
Revision as of 23:44, 18 September 2015 by Vinsonclam (Talk | contribs) (→‎2015 UCLA iGEM)


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Applications of BBa_K1384000

2015 UCLA iGEM

We attempted to use BBa_K1384000 to perform Iterative Capped Assembly, but were unsuccessful. Please see our user review below for more details.

User Reviews

UNIQ469c99783c3548ef-partinfo-00000000-QINU UNIQ469c99783c3548ef-partinfo-00000001-QINU

2015 UCLA iGEM

  • This part does not work as intended. We attempted to use this part to perform ICA, but the other pieces required to perform ICA were not present as part of this collection. In addition, the sticky ends present in this Biobrick are non-specific, and will form undesirable concatemers.
Fig.1 Ligation test using monomer pairs. The presence of laddering bands indicates non-specific binding, showing that this Biobrick is unsuitable for use in ICA.
  • Over the course of summer 2015, we were able to develop a new [http://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics#ICA_Parts_Collection collection of biobricks]. We were able to successfully use this collection to perform ICA.
  • We show below a similar ligation test performed using our parts to examine the ligation behavior of our newly designed sticky ends.