Reporter
GFP-ASV
Part:BBa_K1639023
Designed by: Nurgeldi Bazarov Group: iGEM15_ATOMS-Turkiye (2015-09-18)
Revision as of 23:39, 18 September 2015 by Nurish (Talk | contribs) (ASV tagged GFP is less stable and more useful in researches)
GFP with an ASV degradation tag with a half-life of ~110 min
GFP is seemed to be very stable once produced. But ASV tagged GFP is susceptible to action of intracellular proteases which reduce it's half-life approximately to 110 min. ASV tag consist of C-terminal extension sequences RPAANDENYAASV It's useful for studying of real-time gene expression in individual cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644
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Categories
Parameters
n/a | GFP with an ASV degradation tag with a half-life of ~110 min |