Reporter
GFP-ASV

Part:BBa_K1639023

Designed by: Nurgeldi Bazarov   Group: iGEM15_ATOMS-Turkiye   (2015-09-18)
Revision as of 23:39, 18 September 2015 by Nurish (Talk | contribs) (ASV tagged GFP is less stable and more useful in researches)

GFP with an ASV degradation tag with a half-life of ~110 min

GFP is seemed to be very stable once produced. But ASV tagged GFP is susceptible to action of intracellular proteases which reduce it's half-life approximately to 110 min. ASV tag consist of C-terminal extension sequences RPAANDENYAASV It's useful for studying of real-time gene expression in individual cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


[edit]
Categories
Parameters
n/aGFP with an ASV degradation tag with a half-life of ~110 min