Coding

Part:BBa_K1799007:Design

Designed by: Michael Flanagan   Group: iGEM15_Genspace   (2015-08-07)
Revision as of 23:05, 18 September 2015 by Mjflanagan (Talk | contribs) (→‎Design Notes)


LsrA (modularization optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This version of the open reading frame has been optimized for modularization via silent mutations of the LsrC RBS and ATG, as well as any segments of code sharing more than 83% homology with the Shine-Dalgarno sequence. It is otherwise identical to the wild type LsrA gene (https://parts.igem.org/Part:BBa_K1799003).

Modularization of operon functionality serves a number of important objectives. First, it allows for revised control of individual elements working in concert, including alterations in relative gene expression rates based on prescribed design goals. Modularization enables “copy and paste” excision of individual functionalities such as promoters, ribosome binding sites and coding sequences embedded within the operon. Modularization also allows for precise standardization of functionalities and improved predictability of usage. These last two objectives (excision and precision) were the major focus for our iGEM project involving the LsrACDB operon. The first objective (revision) could not be pursued due to time constraints. However, the excision and revision work accomplished by the Genspace iGEM team serve as an important foundation for future revision pursuits. The LsrACDB operon can be written as shown in the figure below:

Source

This part was synthesized, and is based on E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13806

References