Part:BBa_K1627003
Device to demethylate theophylline
This part contains genes that allow for the demethylation of theophylline. The part contains a synthetic operon consisting of ndmA, ndmB, and ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.
This part can be used in a ∆guaB strain of E. coli to measure the amount of theophylline in a solution. By growing the ∆guaB strain with this device in a minimal media with different known concentrations of theophylline, a growth curve can be made that correlates culture growth with concentration of theophylline. Then if the strain is grown in a minimal media with an unknown concentration of theophylline, the growth can be compared to the growth curve to calculate the initial concentration of theophylline in the media.
Usage and Biology
This device was grown in the ∆guaB strain in minimal media cultures with different concentrations of theophylline. As the concentration of theophylline increases (up to the point of saturation, after which the strain won't grow further), the culture density increases linearly. By comparing growth from unknown concentrations of theophylline to the standard curve, the concentration of theophylline can be measured.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1733
Illegal NheI site found at 1928 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2296
Illegal BglII site found at 2767
Illegal XhoI site found at 3908 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4606
Illegal AgeI site found at 2435 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 983
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