Part:BBa_K1679019

GFP+Promoter+RNA thermometer (FourU) +mRFP

This is a Translational_Unit containing,a RBS (BBa_B0034),a GFP coding sequence (BBa_E0040) ,two terminator(BBa_B0010 and BBa_B0012),a promoter(BBa_J23119K),,a RNA thermometer (BBa_K115003) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli.

There is a RFP reproter device downstream.It has a RNA Thermometers(RNAT) which are temperature-sensing RNA sequences in 5’UTR of their mRNAs. At low temperature, RNAT folds into structure, blocking access of ribosome; at high temperature, RNAT switch from off to open conformation, increasing the efficiency of translation initiation.When the temperature rises above 37°C, RFP would be expressed in theory.

There is a GFP reporter device except promoter in front of the RFP reproter device.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 890
  Illegal NheI site found at 913
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1539
  Illegal AgeI site found at 1651
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 662