Part:BBa_K1679019
GFP+Promoter+RNA thermometer (FourU) +mRFP
This is a Translational_Unit containing,a RBS (BBa_B0034),a GFP coding sequence (BBa_E0040) ,two terminator(BBa_B0010 and BBa_B0012),a promoter(BBa_J23119K),,a RNA thermometer (BBa_K115003) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli. There is a RFP reproter device downstream.It has a RNA Thermometers(RNAT) which are temperature-sensing RNA sequences in 5’UTR of their mRNAs. At low temperature, RNAT folds into structure, blocking access of ribosome; at high temperature, RNAT switch from off to open conformation, increasing the efficiency of translation initiation.When the temperature rises above 37°C, RFP would be expressed in theory. There is a GFP reporter device except promoter in front of the RFP reproter device.
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 890
Illegal NheI site found at 913 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1539
Illegal AgeI site found at 1651 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 662
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