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Part:BBa_K1796008
nifD promoted from Paenibacillus sp. WLY78
Function: αsubunits of dinitrogease which also called FeMo protein. NifD encode a metallocluster: FeMo-co, a [Mo-7Fe-9S-C-homocitrate] cluster which serves as the active site of substrate binding and reduction. We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves(done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But we were not informed of the error in promotion.After several times failed expriments, we found the problem.We tackled them over the matter, mistakes were corrected, but the correct genes can't arrive on time,it can only arrive after the deadline. Sequence submit is promoted by the synthesis company, containing a PstI in the gene.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 150
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 150
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1116
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 150
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 150
Illegal AgeI site found at 1141 - 1000COMPATIBLE WITH RFC[1000]
None |