Device

Part:BBa_K1627004

Designed by: Alejandro Gutierrez   Group: iGEM15_Austin_UTexas   (2015-09-10)
Revision as of 22:28, 18 September 2015 by Algutz (Talk | contribs)

Device to demethylate paraxanthine

This part contains genes that allow for the demethylation of paraxanthine. The part contains a synthetic operon consisting of ndmA, ndmC, and ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.

This part can be used in a ∆guaB strain of E. coli to measure the amount of paraxanthine in a solution. By growing the ∆guaB strain with this device in a minimal media with different known concentrations of paraxanthine, a growth curve can be made that correlates culture growth with concentration of paraxanthine. Then if the strain is grown in a minimal media with an unknown concentration of paraxanthine, the growth can be compared to the growth curve to calculate the initial concentration of paraxanthine in the media.

Usage and Biology

2015 Austin UTexas PDCAFAC growth curve.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1587
    Illegal BglII site found at 2083
    Illegal BglII site found at 2554
    Illegal XhoI site found at 3695
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4393
    Illegal AgeI site found at 2222
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 983


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Categories
Parameters
None