Part:BBa_K1627001

Device to demethylate 3-methylxanthine

This part contains genes that allow for the demethylation of 3-methylxanthine. The part contains a synthetic operon consisting of ndmB, ndmD from Pseudomonas putida CBB5, and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.

This part can be used in a ∆guaB strain of E. coli to measure the amount of 3-methylxanthine in a solution. By growing the ∆guaB strain with this device in a minimal media with different known concentrations of 3-methylxanthine, a growth curve can be made that correlates culture growth (OD600) with concentration of 3-methylxanthine. Then if the strain is grown in a minimal media with an unknown concentration of 3-methylxanthine, the growth can be compared to the growth curve to calculate the initial concentration of 3-methylxanthine in the media.

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 644
  Illegal NheI site found at 839
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1207
  Illegal BglII site found at 1678
  Illegal XhoI site found at 2819
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 3517
  Illegal AgeI site found at 1346
  
• 1000
  COMPATIBLE WITH RFC[1000]