Reporter

Part:BBa_E0422

Designed by: Caitlin Conboy and Jennifer Braff   Group: Antiquity   (2003-12-04)
Revision as of 22:12, 18 September 2015 by SylvieL (Talk | contribs) (→‎Usage and Biology)

ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)

Cyan fluorescent protein with ribosome binding site and terminator


Usage and Biology

This part can be used to test promoter strength or the proper functioning of a regulator device. The LVA tail is particularly useful when testing a regulatory device, for example to assess the efficiency of repressor.

In this experiment, the E0422 part was associated with a cI regulated promoter (part R0051), resulting in K1707028.

Figure 1: Fluorescence microscopy of E. coli DH5alpha bearing K170728

Paris Saclay K1707028 w1POL.png Differential interference contrast

Paris Saclay K1707028 w2Lumencor CFP 434-17.png Excitation with led 437 nm/17nm (CFP), 100 ms of exposure Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/reporter/ret
Parameters
emissioncyan
excitation
tagLVA