Part:BBa_K1642010

Expression of Halorhodopsin under the control of PcpcG2

PcpcG2-HR is a plasmid used to test whether the protein halorhodopsin works as we expect.

Up and Down are sequences used for homologous recombination.

PcpcG2 is the promoter of cpcG2. CcaS phosphorylates CcaR under green light, and phosphorylated CcaR binds to promoter of cpcG2 to induce expression of cpcG2.

RBS is the RNA binding site, which will hopefully increase the expression level of HR.

Halorhodopsin (HR) proteins are light-driven inward-directed chloride pumps from halobacteria. We use this as our biodesalination driver which confers cyanobacteria the ability to absorb chloride to a significant degree.

Terminator is the transcription terminator for the E.coli RNA polymerase.

PcpcB is a constitutive promoter in cyanobacteria, and Kana is gene sequence for kanamycin resistance, so that bacteria which are successfully transformed this plasmid can be screened on the plate.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 3398
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 481
  Illegal NgoMIV site found at 966
  Illegal NgoMIV site found at 1125
  Illegal NgoMIV site found at 1446
  Illegal AgeI site found at 1317
  
• 1000
  COMPATIBLE WITH RFC[1000]