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Applications of BBa_K1642011

Figure1. Control mechanism of Pdark. (a) Under natural light, the green light component induces phosphorylation of CcaR, and the binding of it to Pdark prevents the binding of RNA polymerase, thus inhibiting expression of halorhodopsin. (b) Under darkness, RNA Polymerase binds to Pdark as it does in E.coli and initiate the transcription of halorhodopsin.

The effectiveness of this biodesalination process is proved by determination of the concentrations of extracelluar sodium and chloride or desalination assay, which are shown in Figure 4. During the early time of working stage, there is an obvious decrease of concention compared to that of wild-type, which proves the function of our biobrick(Park-HR, BBa_K1642011). An obvious decrease during the early time and a following rise are consistent with that of the process controlled by PcpcG2.

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To figure out the limitation of desalination, we prolonged the length of the induction stage and adjusted the times of taking samples. The results are shown in Figure 5. The 6h in the working stage is approximately the minimum point.

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The acquisition of the minimum point makes it possible to design a longer biodesalination process. We can extend this process by alternating induction stage (starvation stage) and working stage, make the cyanobacteria to experience starvation and regain of energy for more cycles, thus achieving more reduction of salinity. Moreover, if the length of the induction stage and working stage are approximately 12h, after growth stage this improved biodesalination system can be controlled by the natural alternation between day and night without any human intervention.

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