Part:BBa_K1796103
M Chain of CODH/ACS alpha subunit
The alpha subunit of CODH/ACS have four chains, named M,N,O,P. This four chains have same sequence. CODH/ACS has oxidoreductase function， but this function depend on the intact structure,acluding two subunits.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2210
Illegal EcoRI site found at 1385
Illegal EcoRI site found at 2063 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 1385
Illegal EcoRI site found at 2063
Illegal SpeI site found at 2211
Illegal PstI site found at 2225
Illegal NotI site found at 7
Illegal NotI site found at 2218 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 1385
Illegal EcoRI site found at 2063
Illegal XhoI site found at 1757 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2211
Illegal EcoRI site found at 1385
Illegal EcoRI site found at 2063 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 1385
Illegal EcoRI site found at 2063
Illegal XbaI site found at 16
Illegal SpeI site found at 2211
Illegal PstI site found at 2225
Illegal AgeI site found at 73
Illegal AgeI site found at 1280 - 1000COMPATIBLE WITH RFC[1000]
Parameter of Protein
Number of amino acids: 681
Molecular weight: 79647.4
Theoretical pI: 11.06
Amino acid composition:
Ala (A) 44 6.5%
Arg (R) 121 17.8%
Asn (N) 21 3.1%
Asp (D) 28 4.1%
Cys (C) 30 4.4%
Gln (Q) 17 2.5%
Glu (E) 12 1.8%
Gly (G) 54 7.9%
His (H) 38 5.6%
Ile (I) 12 1.8%
Leu (L) 59 8.7%
Lys (K) 10 1.5%
Met (M) 1 0.1%
Phe (F) 17 2.5%
Pro (P) 66 9.7%
Ser (S) 55 8.1%
Thr (T) 18 2.6%
Trp (W) 17 2.5%
Tyr (Y) 42 6.2%
Val (V) 19 2.8%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 131
Atomic composition:Carbon C 3496
Hydrogen H 5383
Nitrogen N 1185
Oxygen O 915
Sulfur S 31
Formula: C3496H5383N1185O915S31Total number of atoms: 11010
Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 157955
Abs 0.1% (=1 g/l) 1.983, assuming all pairs of Cys residues form cystines
Ext. coefficient 156080
Abs 0.1% (=1 g/l) 1.960, assuming all Cys residues are reduced
Estimated half-life:The N-terminal of the sequence considered is E (Glu).
The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:The instability index (II) is computed to be 74.60
This classifies the protein as unstable.
Aliphatic index: 55.21
Grand average of hydropathicity (GRAVY): -0.984
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