Coding

Part:BBa_K1850012:Design

Designed by: Lydia Goldberg   Group: iGEM15_Harvard_BioDesign   (2015-09-15)
Revision as of 21:56, 18 September 2015 by Lgoldberg (Talk | contribs) (→‎Design Notes)

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fim


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2585
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 868
    Illegal AgeI site found at 899
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

fim was amplified with the native ribosomal binding sites, so that we could maintain the dosing of the fim subunits.

Source

The type I pili structural and transport genes from the fim operon were amplified from the E. coli K-12 genome.

References