Composite

Part:BBa_K1799021

Designed by: Will Shindel, Michael Flanagan   Group: iGEM15_Genspace   (2015-09-17)
Revision as of 21:55, 18 September 2015 by EllenJ (Talk | contribs)

IPTG-inducible generator of RFP

Device was designed and assembled using Synbiota's Rapid DNA Prototyping (RDP) system. Part consists of a Lac promoter, medium RBS, and RFP open reading frame. In LacI- strains, or when cultured in the presence of IPTG, RFP expression is up-regulated. We created this device to use as a control in our experiments testing other devices.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 676
    Illegal AgeI site found at 788
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None