Coding

Part:BBa_K1675003

Designed by: JIAQI XU   Group: iGEM15_BIT-China   (2015-09-09)
Revision as of 21:53, 18 September 2015 by JIAQIXU (Talk | contribs)

glutamate decarboxylase A （GadA）

The gene GadA (glutamate decarboxylase A) can be cloned from Escherichia coli str. K-12 substr. GadA is a functional gene for producing alkali. It catalyzes the proton-consuming conversion of glutamate to γ-amino butyric acid (GABA) whereas it converts glutamate to GABA by removing the α-carboxylate group of glutamate.

The following picture(Fig.1) shows the entire process of gad system.

BIT China Regulation System pic3.png

Fig.1 The mechanism of gadA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
    Illegal AgeI site found at 700
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
Parameters
biologyEscherichia coli str. K-12 substr.
proteinGlutamate Decarboxylase A (GadA)