Composite

Part:BBa_K1799020

Designed by: Will Shindel, Michael Flanagan   Group: iGEM15_Genspace   (2015-09-17)
Revision as of 21:40, 18 September 2015 by EllenJ (Talk | contribs)

Constitutive generator of LacI

Device was designed and assembled using Synbiota's Rapid DNA Prototyping (RDP) system. Device consists of a strong promoter and ribosome binding site, followed by the LacI open reading frame. It is useful for generating the Lac repressor in chassis strains that do not produce it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 47
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None