Part:BBa_K1796012
nifX promoted from Paenibacillus sp. WLY78
Function: encode carrier proteins involved in the delivery of FeMoco to the MoFe protein , participate in FeMoco assembly in a chaperone-like function.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 325
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 325
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 325
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 325
- 1000COMPATIBLE WITH RFC[1000]
Parameter of Protein
Number of amino acids: 136
Molecular weight: 14908.1
Theoretical pI: 6.48
Amino acid composition:
Ala (A) 15 11.0%
Arg (R) 9 6.6%
Asn (N) 4 2.9%
Asp (D) 3 2.2%
Cys (C) 2 1.5%
Gln (Q) 7 5.1%
Glu (E) 13 9.6%
Gly (G) 12 8.8%
His (H) 4 2.9%
Ile (I) 11 8.1%
Leu (L) 10 7.4%
Lys (K) 6 4.4%
Met (M) 5 3.7%
Phe (F) 7 5.1%
Pro (P) 4 2.9%
Ser (S) 8 5.9%
Thr (T) 6 4.4%
Trp (W) 1 0.7%
Tyr (Y) 0 0.0%
Val (V) 9 6.6%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 16
Total number of positively charged residues (Arg + Lys): 15
Atomic composition:Carbon C 655
Hydrogen H 1057
Nitrogen N 189
Oxygen O 194
Sulfur S 7
Formula: C655H1057N189O194S7Total number of atoms: 2102
Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 5625
Abs 0.1% (=1 g/l) 0.377, assuming all pairs of Cys residues form cystines
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 0.369, assuming all Cys residues are reduced
Estimated half-life:The N-terminal of the sequence considered is C (Cys).
The estimated half-life is: 1.2 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:The instability index (II) is computed to be 46.59
This classifies the protein as unstable.
Aliphatic index: 90.44
Grand average of hydropathicity (GRAVY): -0.055
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