Coding

Part:BBa_K1602012

Designed by: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun   Group: iGEM15_TU_Darmstadt   (2015-09-14)
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D-Xylonic acid dehydratases - yjhG

yjhG encodes for a D-xylonic acid dehydratases from E. coli. It catalyzes the reaction of D-xylonic acid to 2-dehydro-3-deoxy-D-pentonate.
https://static.igem.org/mediawiki/parts/f/fe/BBa_K1602012_image_1.png

Characteristics


Molecular Weight 70016.84
Residues 655
Charge -4.0
Isoelectric Point 6.2021
A280 Molar Extinction Coefficients 57870 (reduced) 58370 (cystine bridges)
[Data taken from PEPSTATS]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 998
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1903
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 161
[edit]
Categories
Parameters
None