Coding

Part:BBa_K1796011

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-17)
Revision as of 21:31, 18 September 2015 by Ruifeng (Talk | contribs)

nifN promoted from Paenibacillus sp. WLY78

Function: scaffold gene of nitrogen fixation system

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
    Illegal NgoMIV site found at 648
    Illegal AgeI site found at 25
    Illegal AgeI site found at 147
    Illegal AgeI site found at 766
    Illegal AgeI site found at 987
  • 1000
    COMPATIBLE WITH RFC[1000]


Parameter of Protein

Number of amino acids: 475

Molecular weight: 51557.1

Theoretical pI: 8.13

Amino acid composition: 

Ala (A) 46 9.7%

Arg (R) 31 6.5%

Asn (N) 10 2.1%

Asp (D) 22 4.6%

Cys (C) 8 1.7%

Gln (Q) 14 2.9%

Glu (E) 25 5.3%

Gly (G) 42 8.8%

His (H) 15 3.2%

Ile (I) 21 4.4%

Leu (L) 56 11.8%

Lys (K) 18 3.8%

Met (M) 13 2.7%

Phe (F) 15 3.2%

Pro (P) 24 5.1%

Ser (S) 44 9.3%

Thr (T) 24 5.1%

Trp (W) 8 1.7%

Tyr (Y) 9 1.9%

Val (V) 30 6.3%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 47

Total number of positively charged residues (Arg + Lys): 49

Atomic composition:Carbon C 2282

Hydrogen H 3634

Nitrogen N 648

Oxygen O 671

Sulfur S 21

Formula: C2282H3634N648O671S21Total number of atoms: 7256

Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 57910

Abs 0.1% (=1 g/l) 1.123, assuming all pairs of Cys residues form cystines

Ext. coefficient 57410

Abs 0.1% (=1 g/l) 1.114, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is G (Gly).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 40.14

This classifies the protein as unstable.

Aliphatic index: 91.22

Grand average of hydropathicity (GRAVY): -0.062

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Parameters
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