Generator

Part:BBa_K1758332

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-29)
Revision as of 21:22, 18 September 2015 by Gila (Talk | contribs) (→‎Usage and Biology)

PbrA-5-UTR-sfGFP Lead repressor under control of constitutive promoter and strong RBS

Lead promoter with 5´untranslated reigion and sfGFP for detection


Usage and Biology

For our biosensor we use parts of the chromosomal lead operon of Cupriavidus metallidurans (Ralstonia metallidurans). The promoter that we use is PbrA. This part of the operon is regulated by the repressor pbrR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 133


[edit]
Categories
//function/sensor/metal
Parameters
None