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Part:BBa_K1796009
nifK promoted from Paenibacillus sp. WLY78
Function: β subunits of dinitrogease which also called FeMo protein.NifK encode a metallocluster: the P-cluster, a [8Fe-7S] cluster which shuttles electrons to FeMo-co.
We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves(done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 342
Illegal AgeI site found at 715
Illegal AgeI site found at 1182 - 1000COMPATIBLE WITH RFC[1000]
Parameter of Protein
Number of amino acids: 516
Molecular weight: 56965.8
Theoretical pI: 5.55
Amino acid composition:
Ala (A) 49 9.5%
Arg (R) 28 5.4%
Asn (N) 15 2.9%
Asp (D) 29 5.6%
Cys (C) 9 1.7%
Gln (Q) 19 3.7%
Glu (E) 36 7.0%
Gly (G) 43 8.3%
His (H) 17 3.3%
Ile (I) 24 4.7%
Leu (L) 50 9.7%
Lys (K) 21 4.1%
Met (M) 19 3.7%
Phe (F) 23 4.5%
Pro (P) 26 5.0%
Ser (S) 31 6.0%
Thr (T) 28 5.4%
Trp (W) 2 0.4%
Tyr (Y) 19 3.7%
Val (V) 28 5.4%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 49
Atomic composition:Carbon C 2521
Hydrogen H 3935
Nitrogen N 691
Oxygen O 759
Sulfur S 28
Formula: C2521H3935N691O759S28Total number of atoms: 7934
Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 39810
Abs 0.1% (=1 g/l) 0.699, assuming all pairs of Cys residues form cystines
Ext. coefficient 39310
Abs 0.1% (=1 g/l) 0.690, assuming all Cys residues are reduced
Estimated half-life:The N-terminal of the sequence considered is A (Ala).
The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:The instability index (II) is computed to be 44.53
This classifies the protein as unstable.
Aliphatic index: 81.16
Grand average of hydropathicity (GRAVY): -0.216
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