Coding

Part:BBa_K1675000:Design

Designed by: JIAQI XU   Group: iGEM15_BIT-China   (2015-09-09)
Revision as of 21:01, 18 September 2015 by JIAQIXU (Talk | contribs) (→‎References)


NhaA; sodion-proton antiporter; membrane protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 261
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 458
    Illegal BamHI site found at 995
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 321
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=17489&to=18655

References

[1] E. Padan, M. Venturi, Y. Gerchman, N. Dover, Biochim. Biophys. Acta 1505, 144 (2001). [2] E. Padan et al., Biochim. Biophys. Acta 1658, 2 (2004). [3] A. Rothman, E. Padan, S. Schuldiner, J. Biol. Chem. 271, 32288 (1996). [4] D. Taglicht, E. Padan, S. Schuldiner, J. Biol. Chem. 268, 5382 (1993). [5] S. Schuldiner, H. Fishkes, Biochemistry 17, 706 (1978). [6] Dudu A, Abraham R, Maral B, et al. NhaA Na+/H+ Antiporter Mutants That Hardly React to the Membrane Potential[J]. Plos One, 2014, 9(4):e93200.