Coding

Part:BBa_K1796007

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-17)
Revision as of 21:00, 18 September 2015 by Ruifeng (Talk | contribs)

nifB from Paenibacillus sp. WLY78

essential for biosynthesis of the active-site nitrogenase cofactor and encodes a radical A-adenosylmethionine(SAM)-dependent enzyme that inserts the central carbon atom into the eight-Fe core of nifB cofactor(nifB-co).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1156
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1156
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1156
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1156
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1156
    Illegal AgeI site found at 129
    Illegal AgeI site found at 1089
    Illegal AgeI site found at 1444
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1261


Parametre of Protein

Number of amino acids: 1524

Molecular weight: 124814.2

Theoretical pI: 4.97

Amino acid composition:

Ala (A) 403 26.4%

Arg (R) 0 0.0%

Asn (N) 0 0.0%

Asp (D) 0 0.0%

Cys (C) 365 24.0%

Gln (Q) 0 0.0%

Glu (E) 0 0.0%

Gly (G) 407 26.7%

His (H) 0 0.0%

Ile (I) 0 0.0%

Leu (L) 0 0.0%

Lys (K) 0 0.0%

Met (M) 0 0.0%

Phe (F) 0 0.0%

Pro (P) 0 0.0%

Ser (S) 0 0.0%

Thr (T) 349 22.9%

Trp (W) 0 0.0%

Tyr (Y) 0 0.0%

Val (V) 0 0.0%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 0

Total number of positively charged residues (Arg + Lys): 0

Atomic composition:Carbon C 4514

Hydrogen H 7506

Nitrogen N 1524

Oxygen O 1874

Sulfur S 365

Formula: C4514H7506N1524O1874S365Total number of atoms: 15783

Extinction coefficients:This protein does not contain any Trp residues. Experience shows that

this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 22750

Abs 0.1% (=1 g/l) 0.182, assuming all pairs of Cys residues form cystines

Ext. coefficient 0

Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 39.16

This classifies the protein as stable.

Aliphatic index: 26.44

Grand average of hydropathicity (GRAVY): 0.808

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