Part:BBa_K1587003

3-hydroxybutyryl-CoA dehydratase

Coding region for the 3-hydroxybutyryl-CoA dehydratase (Crt), an enzyme involved in the butyrate biosynthesis pathway of Clostridium acetobutylicum. This substrate of this enzyme is 3-hydroxybutyryl-coA and the product is Crotonyl-CoA (Figure 1).

Figure 1: Crotonyl-CoA formation catalyzed by Crt

We used BBa_K1587003 with other genes to produce butyrate in Escherichia coli (Figure 2).

Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in E. coli

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 609
  
• 1000
  COMPATIBLE WITH RFC[1000]