Coding

Part:BBa_K1800003:Design

Designed by: Laura Lee Irvin   Group: iGEM15_Georgia_State   (2015-08-28)
Revision as of 20:34, 18 September 2015 by Jwhitley5 (Talk | contribs) (→‎Source)


CBDA Synthase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1271
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 541
    Illegal NgoMIV site found at 1120
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was codon optimized for the tobacco plant strain Nicotiana tabacum using the codon optimization tool from IDT. Added was RFC 10 biobrick prefix and suffix. EcoRI, XbaI, SpeI, PstI restriction sites were removed from the coding sequence for iGEM compatibility. Includes native start codon.

Source

Pre-optimized Ssequence from Futoshi et.al, 2007

Synthesized by IDT

References

Taura, Futoshi, et al. "Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa." FEBS letters 581.16 (2007): 2929-2934.