Coding

Part:BBa_K1800003:Design

Designed by: Laura Lee Irvin   Group: iGEM15_Georgia_State   (2015-08-28)
Revision as of 20:28, 18 September 2015 by Jwhitley5 (Talk | contribs) (→‎Design Notes)


CBDA Synthase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1271
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 541
    Illegal NgoMIV site found at 1120
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was codon optimized for the tobacco plant strain Nicotiana tabacum using the codon optimization tool from IDT. Added was RFC 10 biobrick prefix and suffix for iGEM compatibility. EcoRI, XbaI, SpeI, PstI restriction sites were removed from the coding sequence. Includes native start codon.

Source

Synthesized by IDT

References