Part:BBa_K1725020
SrpR repressible promoter
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 20
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
This promoter was characterised by using it to express GFP with two different Ribosome Binding Sites, as found in the BioBricks BBa_E5501 (GFP with B0032 RBS) and BBa_I13500 (GFP with B0034).
GFP fluorescence of K1725001 (PhlF repressible promoter.I13500), K1725002 (PhlF repressible promoter.E5501), K1725021 (K1725020.I13500), K1725022 (K1725020.E5501), K1725082 (TetR repressible promoter.I13500), and E5504 (TetR repressible promoter.E5501) with plasmid backbone pSB3K3 was measured to compare the relative strengths of promoters K1725000 (PhlF repressible promoter) and K1725020 to a promoter already well documented in the registry, R0040 (TetR repressible promoter). Figure 1 the fluorescence scan image and a graph of approximate molecules of GFP per cell. These results indicated that K1725000 is a significantly stronger promoter than R0040 or K1725020.
Figure 1. All constructs with pSB3K3 plasmid backbone, in DH5α cells. Replicates of constructs and controls from three colonies, under the same conditions. Mean and standard deviation of replicates were calculated to give value and error bars.
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