Part:BBa_K1739002

Sequence coding for Sup35 with a N-terminal CsgA signal sequence for amyloid export This part uses the promoter BBa_J23104 and has been inserted into the pSB1C3 backbone. The design includes the bipartite csgA signal sequence that targets the protein to the Sec-export pathway and subsequently to the Curli export pathway via interaction with csgG (Sivanathan and Hochschild, 2012; Sivanathan and Hochschild, 2013). Sup35-NM is derived from the yeast prion protein Sup35p and excludes the C-terminal domain with the N-terminal domain allowing self-assembly of functional amyloid (Frederick et al., 2014; Glover et al. 1997). This has previously been discussed by Tessier and Lindquist (2009) who show that two beta-sheets bond together in a self-complimenting ‘steric zipper’ that excludes water, leaving a highly stable parallel beta-sheet with one molecule every 4.7 Angstroms. The particular advantage of using Sup35-NM is that in its native state Sup35p has two functional domains, the N and C terminal, separated by the highly charged M domain (Frederick et al., 2014; Glover et al. 1997; Wickner et al., 2007) allowing the fusion of a new functional domain.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 718
  

Validation

To validate the nature of our part, we first analysed it using gel electrophoresis. We carried out a diagnostic restriction digest using ECORI and PSTI. These enzymes cleave pSBIC3 into a fragment of 2029bp, whereas our insert is 1020bp. We used the gel to check the sizes of our fragments against the Invitrogen 1kB plus DNA marker (see Fig 1.). We were therefore able to confirm that we had produced the correct part.

Figure 1. Agarose gel of the restriction digest of BBa_K1739002 in pSCB13 plasmid backbone with EcoRI and PstI.

This BioBrick has been validated using a diagnostic Congo Red agar plate. Our plasmid was transformed into the E.coli strain VS45 and compared to a negative control strain, VS45 with pVS105. pVS105 contains CsgA_ss with Sup35M, this protein will not produce amyloid. The results (shown in Fig.2) confirmed that our plasmid induced amyloid formation due to the growth of red colonies of VS45 with pVS72 in comparison to the white VS45 with pVS105, demonstrating no presence of amyloid nano-wires.

Figure 2. Shows our Congo red plates. The top plate depicts both our negative control plasmid, PVS105 and our BioBrick containing CsgAss and Sup35NM.

Further validation was achieved by observing the topography of our cell suspension using Atomic Force Microscopy (AFM). As shown in both Figures 3 and 4, there is clear amyloid formation in the induced VS45 sample. In contrast, there was no amyloid formation in the sample containing the negative control strain VS45 with pVS105 (see figure 5).

Figure 3. An AFM image illustrating the production of nano-wires by our cells containing CsgA_ss and Sup35NM.

Figure 4. An AFM image illustrating the production of nano-wires by our cells containing CsgA_ss and Sup35NM. This image has zoomed in on the cell displaying the nano-wires as seen in Fig. 3.

Figure 5. Shows AFM imaging of our negative control. No nano-wires are present.

The combination of these results demonstrates that our BioBrick facilitates the export of Sup35NM and the subsequent formation of amyloid.

References

Frederick, K., Debelouchina, G., Kayatekin, C., Dorminy, T., Jacavone, A., Griffin, R. and Lindquist, S. (2014). Distinct Prion Strains Are Defined by Amyloid Core Structure and Chaperone Binding Site Dynamics. Chemistry & Biology, 21(2), pp.295-305.

Glover, J., Kowal, A., Schirmer, E., Patino, M., Liu, J. and Lindquist, S. (1997). Self-Seeded Fibers Formed by Sup35, the Protein Determinant of [PSI+], a Heritable Prion-like Factor of S. cerevisiae. Cell, 89(5), pp.811-819.

Sivanathan, V. and Hochschild, A. (2012). Generating extracellular amyloid aggregates using E. coli cells. Genes & Development, 26(23), pp.2659-2667

Sivanathan, V. and Hochschild, A. (2013). A bacterial export system for generating extracellular amyloid aggregates. Nat Protoc, 8(7), pp.1381-1390.

Tessier, P. and Lindquist, S. (2009). Unraveling infectious structures, strain variants and species barriers for the yeast prion [PSI+]. Nat Struct Mol Biol, 16(6), pp.598-605.

Wickner, R., Edskes, H., Shewmaker, F. and Nakayashiki, T. (2007). Prions of fungi: inherited structures and biological roles. Nature Reviews Microbiology, 5(8), pp.611-618.