Part:BBa_K1758333

const. prom+PbrR+PbrA-UTR-sfGFP Lead repressor under control of constitutive promoter and strong RBS Lead repressor under the control of a constitutive promoter with lead induceble promoter and 5´untranslated region in front of a sfGFP for detection.

Usage and Biology

For our biosensor we use parts of the chromosomal lead operon of Cupriavidus metallidurans (Ralstonia metallidurans). The promoter that we use is PbrA. This part of the operon is regulated by the repressor pbrR. The PbrR protein mediates Pb²⁺-inducible transcription. PbrR belongs to the MerR family, which are metal-sensing regulatory proteins (Borremans et al., 2001). Our sensor system is comprised of PbrR, which is under the control of a constitutive promoter and PbrA as well as a 5’ untranslated region, which controls the transcription of a sfGFP. The sfGFP protein is the measuring output signal.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 465
  Illegal NheI site found at 488
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]