Coding

Part:BBa_K1864000

Designed by: Ruicheng Dai   Group: iGEM15_FAFU-CHINA   (2015-09-12)
Revision as of 19:25, 18 September 2015 by Mizzle (Talk | contribs)

CSBV-RdRP

Our part is the gene of CSBV's RNA dependent RNA polymerase(RdRp). We transferred the gene of RdRp into plasmid L4440 ,and transformed the recombinant plasmid into E.coli strain HT115 to express double stranded RNA of RdRp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 837
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Application

Group: FAFU-CHINA

Author: Ruicheng Dai & Changlong Lu


Summary: The control efficiency of dsRdRp in Chinese Scabrood Virus(CSBV)

Design

In our project,we have been aiming to control Chinese Sacbrood virus through silence of CSBV’s RdRp (RNA-dependent RNA polymerase) gene by using RNA-interference technology. We built recombinant L4440 plasmid containing dual T7 promoter and the gene of RNA Dependent RNA polymerase, and we transformed it to HT115 strain. After the enlarge cultivation of HT115, we added the engineered bacteria to the forage of honeybees. To test the effect of dsRdRp, we then did a infectious experiment by feeding infected hives with dsRdRp, and collected data to study the pupation rate of infected hives under different concentrations.



We expected this part to express homologous double-stranded RNA of RdRp. And the result of infectious experiment indicated that this part works as expected.

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