Coding

Part:BBa_K1763000

Designed by: Daniel Cancilla   Group: iGEM15_UCLA   (2015-05-21)
Revision as of 18:48, 18 September 2015 by Dcancilla (Talk | contribs) (→‎Characterization)

Apis mellifera (honeybee) silk fibroin 3

This is the coding region for the honeybee silk protein #3 (will update more later)


Usage and Biology

Hello

Characterization

  1. Protein Expression
  • Here is the SDS gel of our first attempt at expression of the honeybee silk protein. The purified protein should be in the lane labeled "inclusion body." It was purified using [http://wolfson.huji.ac.il/purification/PDF/Protein_Expression_Extraction/NOVAGEN_BugBuster_protein_extraction.pdf this inclusion body protocol]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None