Generator

Part:BBa_K1713015

Designed by: Keji Yan   Group: iGEM15_HZAU-China   (2015-09-14)
Revision as of 18:21, 18 September 2015 by Luoxudong (Talk | contribs)

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luxpR+B0034+LuxI(-LVA)+B0032+AiiA(-LVA)

Expresses Aiia and LuxI proteins. Aiia can hydrolyze AHL internal AHL. LuxI can active the synthetic of AHL. And the expression of LuxI and Aiia are regulated by the concentration of AHL. The promote LuxPR is regulated by the concentration of AHL (N-Acyl homoserine lactone). Aiia is a kind of enzyme could hydrolyze AHL. LuxI is a kind of enzyme actives the synthetic of AHL. LuxI's RBS is B0034, Aiia's RBS is B0032. LVA is a degradation tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 850
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None