Composite

Part:BBa_K1795001

Designed by: Andrew D Halleran, Joseph L Maniaci   Group: iGEM15_William_and_Mary   (2015-09-17)
Revision as of 18:20, 18 September 2015 by Jpmarken (Talk | contribs)

E. Coli Codon Optimized fdCAS9 under R0010

This sequence codes for dCas9 and is codon optimized for E. Coli. It is under the promoter R0010 and has RBS B0034 and DT B0015.

When co-transformed with RFP and BBa_K1795002 97% reduction of RFP was observed. [1] File:WMgRNACompare.png Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1016
    Illegal PstI site found at 2642
    Illegal PstI site found at 3884
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1016
    Illegal PstI site found at 2642
    Illegal PstI site found at 3884
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1216
    Illegal BglII site found at 1272
    Illegal BglII site found at 1414
    Illegal BglII site found at 1962
    Illegal BglII site found at 3802
    Illegal BglII site found at 4041
    Illegal BamHI site found at 3604
    Illegal BamHI site found at 3965
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1016
    Illegal PstI site found at 2642
    Illegal PstI site found at 3884
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1016
    Illegal PstI site found at 2642
    Illegal PstI site found at 3884
    Illegal NgoMIV site found at 4186
    Illegal AgeI site found at 3530
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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