Coding

Part:BBa_K1689001

Designed by: ZHANG Yihao   Group: iGEM15_Peking   (2015-09-16)
Revision as of 18:15, 18 September 2015 by Moximonx (Talk | contribs)

Coding sequence of STV-N-luc

Design

2015 Peking iGEM combines the homotetrameric streptavidin (STV) protein with the N terminal 416 amino acids of firefly luciferase through a GGGGSGGGGS linker. Biotin-STV interaction is one of the strongest noncovalent interactions in the nature, and is frequently applied as a simple approach to generate semisynthetic DNA-protein conjugates. [1] Split luciferase is particularly useful as split bioluminescent reporter for studying protein-protein interaction. [2] In our dual molecular beacons (MB) detecting system, the fusion protein was incubated with biotinylated MB. And when two parts of split luciferase get closed to each other, they will catalyze the luciferin oxidation to produce luminescent signal that could be detected by microplate reader. [1]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter/light
Parameters
None